HeLa cells contain the atrial natriuretic peptide receptor with guanylate cyclase activity

Abstract

Receptors for atrial natriuretic peptide (ANP) are heterogeneous: an ≈140-kDa receptor exhibits ANP-stimulated guanylate cyclase activity whereas an ≈65-kDa receptor is thought to act only as a clearancestorage protein. We have used photoaffinity labeling techniques to show that the human cell line, HeLa, contains predominantly the ≈140-kDa ANP receptor. In contrast, several other cell lines contain primarily the ≈65-kDa receptor. In HeLa cells, ANP bound specifically to high affinity binding sites (Kd≈2 nM) and stimulated a rapid, dose-dependent accumulation of cGMP. These cell lines can thus provide useful models to study the multiple mechanisms of ANP action.