Abstract
BackgroundArachidonic acid (ARA), which is a ω-6 polyunsaturated fatty acid, has a wide range of biological activities and is an essential component of cellular membranes in some human tissues. Mortierella alpina is the best strain for industrial production of ARA. To increase its yield of arachidonic acid, heavy ion beam irradiation mutagenesis of Mortierella alpina was carried out in combination with triclosan and octyl gallate treatment.ResultsThe obtained mutant strain F-23 ultimately achieved an ARA yield of 5.26 g L− 1, which is 3.24 times higher than that of the wild-type strain. In addition, quantitative real-time PCR confirmed that the expression levels of fatty acid synthase (FAS), Δ5-desaturase, Δ6-desaturase, and Δ9-desaturase were all significantly up-regulated in the mutant F-23 strain, especially Δ6- and Δ9-desaturase, which were up-regulated 3- and 2-fold, respectively.ConclusionsThis study confirmed a feasible mutagenesis breeding strategy for improving ARA production and provided a mutant of Mortierella alpina with high ARA yield.
Highlights
Arachidonic acid (ARA), which is a ω-6 polyunsaturated fatty acid, has a wide range of biological activities and is an essential component of cellular membranes in some human tissues
Heavy ion beam irradiation-induced M. alpina mortality Heavy ion mutagenesis has been successfully applied to plants [27, 28] and some industrial microbial mutagenesis breeding programs [11, 29], but as far as we know, this study is the first to use it with M. alpina
Due to a lack of reference conditions for the heavy ion mutagenesis of M. alpina, we investigated the lethality of five doses of heavy ion beam irradiation in this experiment
Summary
Arachidonic acid (ARA), which is a ω-6 polyunsaturated fatty acid, has a wide range of biological activities and is an essential component of cellular membranes in some human tissues. Mortierella alpina is the best strain for industrial production of ARA. To increase its yield of arachidonic acid, heavy ion beam irradiation mutagenesis of Mortierella alpina was carried out in combination with triclosan and octyl gallate treatment. Arachidonic acid (ARA), which is a ω-6 polyunsaturated fatty acid, has a wide range of biological activities as it is a prerequisite for the synthesis of prostaglandins, thromboxane and leukotrienes [1]. At present, the genetic engineering method has been widely used in the increase of ARA production of M. alpina [6,7,8]. As a chemical mutagenesis method, 5-fluorouracil (5-FU) is a structural analog of uracil that inhibits the synthesis of DNA and some RNA [14, 15], and it has been successfully used for
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