Abstract
The st mutation in the T5 wild-type phage, demonstrated by an increased heat stability of the phage particle, causes changes in the T5 DNA molecule. The five different T5st mutants studied have 2–7% less DNA than the wild-type phage. When the wild-type T5 DNA molecule is first broken by shear it breaks asymmetrically into long and short fragments representing 65 and 35% of the mass of the molecule. When the DNA molecule from the T5st mutants is first broken by shear, it also breaks asymmetrically with a decrease in the average size of the long fragments and an increase in the average size of the short fragments. These changes are specific for each of the st mutants. Other physical parameters of the phage parallel their loss of DNA. Those phage that have lost the greatest amount of DNA are the most heat stable, sediment the slowest, and have the lowest density.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
