Abstract
Highly consistent results were obtained in banding of chromosomes with the 2xSSC method of Sumner et al. modified by the use of preheated Giemsa staining solution at 40–45°C. A 4xSSC method modified in the same way turned out to give the same consistent banding on flame-dried chromosomal specimens. Temperature seems to be a decisive factor in the band-producing capacity of a given Giemsa dye.
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