Abstract
A silicalite-1 film (SF) deposited on Ti-6Al-4V alloy was investigated in this study as a promising coating for metallic implants. Two forms of SFs were prepared: as-synthesized SFs (SF-RT), and SFs heated up to 500 °C (SF-500) to remove the excess of template species from the SF surface. The SFs were characterized in detail by X-ray photoelectron spectroscopy (XPS), by Fourier transform infrared spectroscopy (FTIR), by scanning electron microscopy (SEM) and water contact angle measurements (WCA). Two types of bone-derived cells (hFOB 1.19 non-tumor fetal osteoblast cell line and U-2 OS osteosarcoma cell line) were used for a biocompatibility assessment. The initial adhesion of hFOB 1.19 cells, evaluated by cell numbers and cell spreading area, was better supported by SF-500 than by SF-RT. While no increase in cell membrane damage, in ROS generation and in TNF-alpha secretion of bone-derived cells grown on both SFs was found, gamma H2AX staining revealed an elevated DNA damage response of U-2 OS cells grown on heat-treated samples (SF-500). This study also discusses differences between osteosarcoma cell lines and non-tumor osteoblastic cells, stressing the importance of choosing the right cell type model.
Highlights
A silicalite-1 film (SF) deposited on Ti-6Al-4V alloy was investigated in this study as a promising coating for metallic implants
No morphological changes were induced by calcination of SFs, so the morphology of both samples (SF-room temperature (RT) and SFs heated up to 500 °C (SF-500)) is comparable
Heat treatment of SFs improved the initial adhesion of hFOB 1.19 cells due to the increased wettability of SF-500
Summary
A silicalite-1 film (SF) deposited on Ti-6Al-4V alloy was investigated in this study as a promising coating for metallic implants. High biocompatibility of implant materials with the tissue and with the body fluids is required for good integration and healing without undesirable effects[5] Because of their superior mechanical properties, metallic alloys are the most widely-used materials for the fabrication of load-bearing orthopedic implants[3,4]. MFI zeolites (such as aluminosilicate ZSM-5 and pure-silica silicalite-1) are very attractive materials for coating implants, due to their high thermal and chemical stability[22] together with their high wear and corrosion resistance[23,24,25] Another important advantage is the low elastic modulus of MFI films, which can match the modulus of the host cortical bone (about 30 GPa, while the modulus of bare Ti-6Al-4 V alloy is about 110 GPa24,26). A similar increase in DNA damage was observed in cells grown on the reference stainless steel without SFs
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