Abstract
SummaryHuman sera, covering a wide range of total IgE concentrations, were subjected to mild heat treatment for varying periods of time and then examined for their abilities to bind to an Fc‐specific, radioactively labelled rabbit anti‐human IgE serum. As a result of the heat treatment, aggregation of the serum IgE occurred and the aggregated material reacted significantly with the labelled antiserum. Experiments with a standard human IgE serum and over 100 different human serum samples from normal and allergic patients showed that this simple heat method can distinguish high, medium and low levels of IgE in serum. Provided that sufficient examinations are undertaken, the method offers a simple means of rapidly examining large numbers of serum samples and distinguishing those sera with elevated levels of IgE.Differences observed between individual serum samples may indicate physico‐chemical differences in the IgE molecules present in the sera. Such differences may be a further reflection of the heterogeneity of human IgE. The stability of human skin‐sensitizing antibodies is briefly reviewed.
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