Heat stress-responsive transcriptome analysis in heat susceptible and tolerant wheat (Triticum aestivum L.) by using Wheat Genome Array

Dandan Qin,Qixin Sun,Haiyan Wu,Chunlei Zhou,Zhenxing Li,Huiru Peng,Zhongfu Ni,Yingyin Yao

doi:10.1186/1471-2164-9-432

Abstract

BackgroundWheat is a major crop in the world, and the high temperature stress can reduce the yield of wheat by as much as 15%. The molecular changes in response to heat stress are poorly understood. Using GeneChip® Wheat Genome Array, we analyzed genome-wide gene expression profiles in the leaves of two wheat genotypes, namely, heat susceptible 'Chinese Spring' (CS) and heat tolerant 'TAM107' (TAM).ResultsA total of 6560 (~10.7%) probe sets displayed 2-fold or more changes in expression in at least one heat treatment (false discovery rate, FDR, α = 0.001). Except for heat shock protein (HSP) and heat shock factor (HSF) genes, these putative heat responsive genes encode transcription factors and proteins involved in phytohormone biosynthesis/signaling, calcium and sugar signal pathways, RNA metabolism, ribosomal proteins, primary and secondary metabolisms, as well as proteins related to other stresses. A total of 313 probe sets were differentially expressed between the two genotypes, which could be responsible for the difference in heat tolerance of the two genotypes. Moreover, 1314 were differentially expressed between the heat treatments with and without pre-acclimation, and 4533 were differentially expressed between short and prolonged heat treatments.ConclusionThe differences in heat tolerance in different wheat genotypes may be associated with multiple processes and mechanisms involving HSPs, transcription factors, and other stress related genes. Heat acclimation has little effects on gene expression under prolonged treatments but affects gene expression in wheat under short-term heat stress. The heat stress responsive genes identified in this study will facilitate our understanding of molecular basis for heat tolerance in different wheat genotypes and future improvement of heat tolerance in wheat and other cereals.

Highlights

Wheat is a major crop in the world, and the high temperature stress can reduce the yield of wheat by as much as 15%
We found that the expression levels of 41 probe sets for tRNAs were increased during 24-h heat treatments, suggesting an increased level of protein biosynthesis during the longterm heat stress
We found that the heat – responsive (HR) genes include those encoding putative NADPH oxidase, mitochondrial alternative oxidase (AOX), germin-like oxalate oxidases and amine oxidases, which are related to reactive oxygen species (ROS) production [69]

Summary

Introduction

Wheat is a major crop in the world, and the high temperature stress can reduce the yield of wheat by as much as 15%. The high temperatures during the post-heading stages affect yield [4,5] and grain quality [6] of wheat, a major crop cultivated worldwide. If plants are pretreated with a mild non-lethal temperature (heat acclimation) or if temperature increases gradually to a lethal level, they can survive under the lethal high temperature stress, which is known as acquired thermotolerance [7,8]. Little is known about the molecular changes affecting regulatory and biochemical pathways of heat stress responses in crops [9]. Identifying novel genes and studying their expression patterns in response to heat stress will provide a molecular basis for improving heat tolerance in crops

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Results

Conclusion