Heat stress of two tropical seagrass species during low tides - impact on underwater net photosynthesis, dark respiration and diel insitu internal aeration.

Abstract

Seagrasses grow submerged in aerated seawater but often in low O2 sediments. Elevated temperatures and low O2 are stress factors. Internal aeration was measured in two tropical seagrasses, Thalassia hemprichii and Enhalus acoroides, growing with extreme tides and diel temperature amplitudes. Temperature effects on net photosynthesis (PN ) and dark respiration (RD ) of leaves were evaluated. Daytime low tide was characterized by high pO2 (54 kPa), pH (8.8) and temperature (38°C) in shallow pools. As PN was maximum at 33°C (9.1 and 7.2μmol O2 m(-2) s(-1) in T.hemprichii and E.acoroides, respectively), the high temperatures and reduced CO2 would have diminished PN , whereas RD increased (Q10 of 2.0-2.7) above that at 33°C (0.45 and 0.33μmolO2 m(-2) s(-1) , respectively). During night-time low tides, O2 declined resulting in shoot base anoxia in both species, but incoming water containing c. 20kPa O2 relieved the anoxia. Shoots exposed to 40°C for 4h showed recovery of PN and RD , whereas 45°C resulted in leaf damage. These seagrasses are 'living near the edge', tolerant of current diel O2 and temperature extremes, but if temperatures rise both species may be threatened in this habitat.