Abstract
Heat stress in hot climates is a major cause that negatively affects dairy animals, leading to substantial economic loss. The present study was aimed to analyze the effect of heat stress on cellular and molecular levels in dermal fibroblast of cattle and buffaloes. Primary fibroblast culture was established using ear pinna tissue samples of cattle (Bos indicus) and riverine buffaloes (Bubalus Bubalis). The cells were exposed to thermal stress at 42°C for 1 h and subsequently allowed to recover and harvest at 37°C at different time points (0, 2, 4, 8, 16, and 24 h) along with control samples. Different cellular parameters viz., apoptosis, proliferation, mitochondrial membrane potential (ΔΨm), oxidative stress, along with expression pattern of heat responsive genes and miRNAs were determined. Cell viability and proliferation rate of heat-stressed fibroblasts decreased significantly (P < 0.05) albeit to a different extent in both species. The cell cytotoxicity, apoptosis, production of reactive oxygen species, and ΔΨm increased more significantly (P < 0.01) in heat stressed fibroblasts of buffalo than cattle. The pattern of heat shock proteins, inflammation/immune genes, and heat responsive miRNA showed differences in induction of their expression level in buffalo and native cattle fibroblasts. Conclusively, finding indicates that heat stress induces more profound impact on buffalo fibroblasts than native cattle fibroblasts. The differential response of cellular parameters, HSP genes, and miRNA expression could be due to better adaptive capacity of skin fibroblast of Bos indicus cattle in comparison with riverine buffaloes.
Highlights
Environmental heat stress imposes a significant burden on dairy animal production across the world by adversely affecting their production and reproduction ability
The Lactate dehydrogenase (LDH) release, which is an indicator of cytotoxicity, increased significantly post heat stress and thereafter decreased continuously during the recovery period in Buffalo fibroblast (BFb) cells
cow fibroblast (CFb) had maximum LDH release after 8 h of post-stress (Figure 1A). Though it was relatively lower in comparison with maximum cytotoxicity observed for BFb. These results suggested that heat stress exerts a cytotoxic effect on fibroblast of both native cattle and buffaloes; the extent of cytotoxicity was higher in BFb as compared with CFb indicating their higher susceptibility to heat stress
Summary
Environmental heat stress imposes a significant burden on dairy animal production across the world by adversely affecting their production and reproduction ability. The data indicated heat stressed induced depolarization of ψm in fibroblast cells of both the species; the impact was more in BFb as compared to CFb (Figure 2B).
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