Heat Stress-Induced Cell Death, Changes in Antioxidants, Lipid Peroxidation, and Protease Activity in Wheat Leaves

Abstract

Six wheat genotypes were evaluated for heat tolerance in terms of seedling growth, antioxidant response and cell death. Based on the heat susceptibility index (HSI), response of the genotypes varied from heat tolerant (Inqilab-91) to heat sensitive (Sitta) along with moderately tolerant (Nesser and Sarsabz) and sensitive (Fareed and FD-83). Heat stress-induced programmed cell death (probably necrosis) in wheat leaves was evident by DNA smear. MDA content increased above twofold in most of genotypes under heat stress, with the lowest increase in the heat-tolerant genotype Nesser. Catalase activity diminished under heat stress in all genotypes. Peroxidase, superoxide dismutase (SOD), protease, and ascorbate peroxidase (APX) activities increased under heat stress. Apparently, heat stress-induced reduction in catalase activity was compensated by a parallel increase in peroxidases to quench H2O2. Heat stress-induced decrease (%) in catalase and increase in protease activities showed significant positive correlations, whereas increase (%) in APX activity showed a significant negative correlation with HSI or relative heat tolerance of genotypes. All these correlations signify that catalase, protease and ascorbate peroxidase can be used efficiently as biochemical markers to assess the relative heat stress tolerance of wheat genotypes at the seedling stage. In conclusion, using a multiparametric approach involving morphophysiological and biochemical assays, the sensitivity of wheat genotypes to heat stress could be evaluated to a sufficient level of certainty at the seedling stage.