Abstract
The production of toxic filtrates from various members of the enteric group of organisms has been reported frequently since Pfeiffer1 postulated the theory that an endotoxic substance existed preformed in the cells of this group. Many methods have been used and results have been varied and inconclusive. Chase2 comparing products obtained by various methods, concluded that as toxic filtrates as any resulted from the simplest?that of heating water suspensions of fresh agar cultures at 60 C. for 4 hours and filtering through Berkefeld candles. The present work follows this method. I was particularly interested in comparing filtrates made from cultures with and without the additional Vi antigen described by Felix and Pitt3 and in studying their heat stability and immunologic activities.
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