Abstract
Background: Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the development of bronchopulmonary dysplasia (BPD).Methods: This birth cohort study enrolled 109 VLBW preterm infants, including 32 infants who developed BPD. Hsp-70 and 8-OHdG concentrations from TA were measured by immunoassay. The apoptosis of TA epithelial cells obtained on Day 28 after birth was measured using annexin-V staining assay.Results: Hsp-70 and 8-OHdG levels in TA fluid were persistently increased from Day 1 to Day 28 of life in the BPD group. Multiple linear regression analysis demonstrated that BPD was significantly associated with gestational age, respiratory distress syndrome, and TA Hsp-70 and 8-OHdG levels on post-natal Day 28. The TA Hsp-70 level positively correlated with TA 8-OHdG level on the Day 1 (r = 0.47) and Day 28 of life (r = 0.68). Incubation of recombinant Hsp-70 with primary epithelial cells derived from TA of patients decreased hydrogen peroxide-induced epithelial cell death.Conclusions: Hsp-70 levels are associated with a state of oxidative injury in the development of BPD.
Highlights
Heat shock proteins (Hsps) constitute a family of molecular chaperones constitutively expressed in virtually all nucleated cells
This study aimed to compare the relationship between the Heat shock protein-70 (Hsp-70) and 8-OHdG levels from tracheal aspirates (TA) and the development of Bronchopulmonary dysplasia (BPD) among very low-birth weight (VLBW) infants
To clarify the anti-apoptosis mechanisms involved in the Hsp-70, we examined the apoptosis of respiratory epithelial cells via the propidium iodide (PI)/annexin-V assay from TA of patients with BPD
Summary
Heat shock proteins (Hsps) constitute a family of molecular chaperones constitutively expressed in virtually all nucleated cells. Bronchopulmonary dysplasia (BPD) is the most common chronic lung disease of very low-birth weight (VLBW) preterm infants at risk of mortality [5]. The pathogenesis of BPD is a multifactorial process linked to the arrested development of immature lungs, barotrauma, oxidant injury, inflammation, enlarged airspaces, and mild airway smooth muscle thickening [6,7,8]. Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the development of bronchopulmonary dysplasia (BPD)
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