Abstract
A modification of the plate count method is described which brings important advantages upon the normal plate-counting system as used in heat resistance determinations ( D t). It saves cost and time, and makes less liable the appearance of handling errors. Precision of counting in the range of 70–20000 colonies per plate was studied and D t values obtained by survival counting at three ranges of number of colonies per plate were compared. Precision of this system was shown to be as high as that of the pour plate method as normally used, and no significant difference was found among thermal death rates ( D 105 values) obtained at the three ranges of number of colonies per plate tested.
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