Heat resistance and heat-shock protein synthesis in a reptilian cell line, IgH-2, from Iguana iguana

Abstract

1. 1.Cells of the line IgH-2 from Iguana iguana proliferated at both 22 and 36°C, endured temperatures from 22 to 42°C, died at 0°C and temperatures above 42°C, and survived a 46°C incubation better if they had been grown at 36 than at 22°C. 2. 2.For both 22 and 36° cultures, synthesis of the major heat-shock proteins (hsps) was enhanced abuptly at 42°C, was maximal at 44°C, was reduced significantly at 46°C, and consisted of polypeptides at 87 and 70 kDa, which was similar to other organisms, at 76kDa and at 29, 28, 22 and 21 kDa, which among vertebrates appeared to be unique to IgH-2. 3. 3.Among the minor differences in the heat-shock response of 22 and 36°C cultures was the increased synthesis in 22°C cultures of hsp 87 from 28 to 44°C and of a 39 kDa polypeptide from 36 to 40°C. 4. 4.Tumicamycin and 2-deoxyglucose treatments enhanced the synthesis of polypeptides at 76 and 97 kDa.