Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts.

Félix-Antoine Pellerin,Stéphane Bolduc,Ève Pellerin,Martin Pelletier,Christophe Caneparo,Stéphane Chabaud

doi:10.3390/bioengineering8110184

Félix-Antoine Pellerin, Stéphane Bolduc + Show 4 more

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https://doi.org/10.3390/bioengineering8110184

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Journal: Bioengineering	Publication Date: Nov 13, 2021
Citations: 5	License type: CC BY 4.0

Affiliation: Université Laval

Abstract

Heat inactivation of bovine sera is routinely performed in cell culture laboratories. Nevertheless, it remains debatable whether it is still necessary due to the improvement of the production process of bovine sera. Do the benefits balance the loss of many proteins, such as hormones and growth factors, that are very useful for cell culture? This is even truer in the case of tissue engineering, the processes of which is often very demanding. This balance is examined here, from nine populations of fibroblasts originating from three different organs, by comparing the capacity of adhesion and proliferation of cells, their metabolism, and the capacity to produce the stroma; their histological appearance, thickness, and mechanical properties were also evaluated. Overall, serum inactivation does not appear to provide a significant benefit.

Highlights

Since the second half of the 20th century, molecular and cell biology has been based on the culture of cell lines or primary cell populations
The proliferation and maintenance of the cells are mainly achieved through a cell culture medium containing serum
Part of the growth factors, vitamins, and precious nutrients required for cell culture and activity can be lost during this exposition to heat

Summary

Introduction

Since the second half of the 20th century, molecular and cell biology has been based on the culture of cell lines or primary cell populations. The proliferation and maintenance of the cells are mainly achieved through a cell culture medium containing serum. Before its use, FBS is routinely heat-inactivated, generally at 56 ◦ C for 30 min, mainly to prevent the action of complement components and contamination by mycoplasma [3,4]. Part of the growth factors, vitamins, and precious nutrients required for cell culture and activity can be lost during this exposition to heat. From the beginning of cell culture, there is a gap in technologies to assure the quality of the products provided for cell culture. This is true for the FBS production, storage, and quality assessment [1]. As early as 1980, a study demonstrated that complement components are present in very low or undetectable levels in FBS samples [3]

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