Abstract
Ochratoxin content in sclerotia collected from grains of barley, wheat, and corn inoculated with Aspergillus ochraceus was significantly higher than that accumulated in sclerotia produced on synthetic medium (SM) (95.8, 47.3, 9.2 and 1.75 μg/g sclerotia, respectively). However, when sclerotia harvested from those grains were grown for 14 d on SM the amount of ochratoxin produced by the colonies formed was 3.4, 2.7 and 3.0 μg/plate, as compared with 5.2 μg/plate produced under the same growth conditions by colonies initiated from sclerotia which originated from the SM. Complete mortality of sclerotia from grains or SM occurred at a dose of 200 krad, but none of the levels tested (25, 50 or 100 krad) caused an increase in ochratoxin production. A marked reduction in sclerotial germination was recorded for sclerotia obtained from SM but not for those obtained from wheat grains after exposure to combined treatments of irradiation plus heat. Nevertheless, the combined treatment did not consistently result in significantly different quantities of ochratoxin produced.
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