Abstract
When bean or cowpea leaves were inoculated with certain viruses and, within 8 seconds after mechanical inoculation, held in water or mercury for 0.5-4 seconds at 45–65°, the number of resulting lesions were increased. As the interval from inoculation to heating was increased beyond 10 seconds, heat activation decreased and heat inactivation occurred from 1 minute to 3 hours after inoculation, followed by heat activation again from 5 to 24 hours after inoculation, after which heat had little effect on numbers of lesions. As the duration of heat increased beyond 4 seconds, heat activation decreased and heat inactivation increased. The optimum heating time for increasing the number of lesions, and minimum time for maximum reduction in lesion formation decreased with increasing temperature. The greatest increase in infection from heating inoculated leaves was with the sweet potato strain of tobacco mosaic virus on Pinto bean.
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