Heart-type fatty acid-binding protein and its relation with morphological changes in rat myocardial damage model induced by isoproterenol

Radivoj Jadrić,Sabaheta Hasić,Mira Winterhalter-Jadrić,Zakira Mornjaković,Esad Ćosović,Emina Kiseljaković

doi:10.17305/bjbms.2011.2557

Abstract

We have investigated heart type fatty acid binding protein (H-FABP) rat serum values at different time point following subcutaneous (s.c) isoproterenol (ISO) administration and their correlation with severity of myocardial lesion. Thirty adult, male, Wistar rats were used for this study. Six rats per group were treated with a single dose of either ISO (ISO groups, dose 100 mg/kg, s.c.) at different time point (30', 60', 120', 240') or with saline (control group). Serum H-FABP was determined by enzyme-linked immunosorbent assay (ELISA) and histological analysis was performed by hematoxylin-eosin (HE) method of staining. The first serum H-FABP increase was obtained 30' following ISO administration, but maximal value was reached after 240'. Myocardial histological changes were time-dependent and correlated with serum H-FABP values (p<0.001). The results of the study suggest that H-FABP is sensitive marker for acute rat myocardial injury and its possible inclusion in myocardial injury screening studies in rats.

Highlights

Cardiac biomarkers are protein macromolecules that are found in myocardial cells, and their serum increasing is indicative in cell damage or loss of cell membranes integrity [1, 2]
Mean baseline serum value of rat heart fatty acid binding protein (H-FABP) in the groups was from 0.73-1.35 ng/ ml
ISO administration to rats was followed by H-FABP releasing from cardiomyocytes into circulation

Summary

Introduction

Cardiac biomarkers are protein macromolecules that are found in myocardial cells, and their serum increasing is indicative in cell damage or loss of cell membranes integrity [1, 2]. HFABP is 14.5 kDa protein, consisted of 132 amino acid residues. It comprises 4-8% of the total cytoplasmic protein of cardiac myocytes and its function is hydrophobic long-chain fatty acids transport from the cell membrane to their intracellular sites of metabolism in the mitochondria. As in human, H-FABP is distributed in heart and in skeletal muscles, kidneys, brain but with lower expression [4, 5]. Heart content of H-FABP is approximately 10-fold greater than in most skeletal muscles [6]

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