Healthy baby born after ICSI with ejaculated immotile spermatozoa from a male Kartagener syndrome using Magnetic-Activated Cell Sorting (MACS) as a compliment of sperm preparation technique: A case report

Abstract

Kartagener syndrome is an autosomal recessive genetic disorder associated with male infertility. Mutations in genes that encode a protein called dynein affect sperm motility.A good option for these patients is to enhance the sperm selection for ICSI that could lead to improve the reproductive outcomes. For this purpose, different strategies have been used successfully in isolated cases.In the present case report, we successfully applied MACS technique to a semen sample. This immunomagnetic method of sperm selection was aimed at reducing apoptosis manifestations, including DNA fragmentation, to optimize the outcomes of the ICSI procedure. By doing so, we increased the chances of selecting spermatozoa with superior quality and higher fertilization potential in the presence of total asthenozoospermia, even after incubation with pentoxifylline. Six of ten oocytes (60%) were appropriately fertilized. Two good-quality embryos were transferred on day 3 resulting in a pregnancy and a healthy baby born and one cavitated blastocyst was frozen on day 5.MACS technique used as a compliment of sperm preparation technique may be a promising approach in routine IVF practice for men with Kartagener syndrome to improve reproductive outcomes optimizing the outcomes of the ICSI procedure.