Abstract
We established a novel identification method for oral Rothia species using one-step multiplex PCR analysis to investigate whether the monitoring of oral Rothia species levels is useful for peri-implantitis risk assessment, and to examine the oxygen concentration that these organisms need for growth in vitro. The mean number and proportion of Rothia aeria in peri-implant sulcus fluid (PISF) samples was significantly higher in the healthy implant group than in the peri-implantitis group (P R. aeria under aerobic conditions vigorously grew compared with those under anaerobic conditions, and this organism grew only at the upper layer where high oxygen concentrations existed in a semi-liquid nutrient medium. Therefore, the monitoring of R. aeria levels may be suitable as an indicator of healthy peri-implant tissue conditions for the prevention of peri-implantitis.
Highlights
The successful use of jawbone-anchored titanium dental implants for the rehabilitation of edentulous and partially dentate patients has been well documented [1]-[7]
The specific forward primers were designated as RMFF for R. mucilaginosa, RDFF for R. dentocariosa, and RAFF for R. aeria, whereas the specific reverse primers were designated as RMFR for R. mucilaginosa, RDFR for R. dentocariosa, and RAFR for R. aeria
The detection limit was assessed in the presence of titrated bacterial cells, and the sensitivity of the PCR assay was between 5 × 1 and 5 × 10 colonyforming units (CFU) per PCR template (5.0 μl) for the R. mucilaginosa-specific primer set with strain JCM 10910, the R. dentocariosaspecific primer set with strain JCM 306, and the R. aeria-specific primer set with strain JCM 11412
Summary
The successful use of jawbone-anchored (osseointegrated) titanium dental implants for the rehabilitation of edentulous and partially dentate patients has been well documented [1]-[7]. The results of implant treatment have mostly been satisfactory with survival rates of 85% to 99%. Infections such as implant mucositis and peri-implantitis occur around dental implants [8] [9] [10] [11] [12]. Patients with a history of periodontitis appear to be more susceptible to developing peri-implant infections [14]. Smoking is another risk factor that has been associated with peri-implant infections [15] [16] [17] [18]
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