Abstract
Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats were randomly divided into six groups of six each and were: untreated control, treated with Silverdin® cream, base cream, base cream with c% EGF, base cream with 3% TRF or base cream with c% EGF and 3% TRF, respectively. Creams were applied once daily for 21 consecutive days. Six animals from each group were sacrificed using anaesthetic overdose on the third, seventh, 11th, 14th and 21st day post-burn. Skin tissues with the wound to be examined were excised for macroscopic and microscopic evaluation and biochemical analyses. Results: EGF + TRF formulation decreased the number of neutrophils, lymphocytes and myofibroblasts post-burn. However, no effects on the number of adipose cells in the healing process were recorded. In addition, lipid peroxidation and nitrite production were found to be reduced post-burn, reducing oxidative stress. Conclusions: Results of the present study indicate that the addition of EGF with TRF have ameliorating effects on deep-partial thickness burn healing parameters.
Highlights
Burns are defined as skin lesions and are caused mainly by heat or other acute trauma, including scalds, contact with heating elements and flames
Xylazine and ketamine were obtained from Troy Laboratories (Glendenning, Australia); Tramadol was purchased from CCM Duo
The current study demonstrated that the NO level was significantly reduced on the 14th and 21st days post-burn in the epidermal growth factor (EGF) + tocotrienol-rich fraction (TRF) group, indicating that the EGF + TRF treatment could reduce the oxidative stress at the later stage of wound healing, reducing inflammation in the wound area
Summary
Burns are defined as skin lesions and are caused mainly by heat or other acute trauma, including scalds, contact with heating elements and flames. When lesions penetrate into the dermis, they are denoted as partial-thickness or second-degree burns and further classified as superficial or deep partial-thickness burns [4]. Deep partial-thickness burns can cause deep dermis damage where the skin is drier, the sensation of the skin tends to be weakened and the hair is easy to be sloughed off. Deep partial-thickness burns heal slowly and are accompanied by scar formation and potential functional loss [4]. An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Results: EGF + TRF formulation decreased the number of neutrophils, lymphocytes and myofibroblasts post-burn. Conclusions: Results of the present study indicate that the addition of EGF with TRF have ameliorating effects on deep-partial thickness burn healing parameters
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