Healing Effect of Vicenin-2 (VCN-2) on Human Dermal Fibroblast (HDF) and Development VCN-2 Hydrocolloid Film Based on Alginate as Potential Wound Dressing.

Woan Sean Tan,Sharida Fakurazi,Che Norma Mat Taib,Palanisamy Arulselvan,Shiow-Fern Ng,Murni Nazira Sarian

doi:10.1155/2020/4730858

Woan Sean Tan, Sharida Fakurazi + Show 4 more

Open Access

https://doi.org/10.1155/2020/4730858

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Abstract

Chronic wounds represent serious globally health care and economic issues especially for patients with hyperglycemic condition. Wound dressings have a predominant function in wound treatment; however, the dressings for the long-lasting and non-healing wounds are still a significant challenge in the wound care management market. Astonishingly, advanced wound dressing which is embedded with a synthetic drug compound in a natural polymer compound that acts as drug release carrier has brought about promising treatment effect toward injured wound. In the current study, results have shown that Vicenin-2 (VCN-2) compound in low concentration significantly enhanced cell proliferation and migration of HDF. It also regulated the production of pro-inflammatory cytokines such as IL-6, IL-1β, and TNF-α from HDF in wound repair. Treatment of VCN-2 also has facilitated the expression of TGF-1β and VEGF wound healing maker in a dose-dependent manner. A hydrocolloid film based on sodium alginate (SA) incorporated with VCN-2 synthetic compound which targets to promote wound healing particularly in diabetic condition was successfully developed and optimized for its physico-chemical properties. It was discovered that all the fabricated film formulations prepared were smooth, translucent, and good with flexibility. The thickness and weight of the formulations were also found to be uniform. The hydrophilic polymer comprised of VCN-2 were shown to possess desirable wound dressing properties and superior mechanical characteristics. The drug release profiles have revealed hydrocolloid film, which is able to control and sustain the VCN-2 released to wound area. In short, hydrocolloid films consisting of VCN-2 formulations are suitably used as a potential wound dressing to promote restoration of wound injury.

Highlights

Wound healing is a well-programmed complex process which involves inflammation, cell migration and proliferation, and neovascularization extracellular matrix production and remodeling [1]
The results showed that VCN-2 at lower concentrations have increased cell viability while at higher concentrations have reduced the cell viability
All dosages of VCN-2 used in this experiment did not exhibit any toxic effect to human dermal fibroblast (HDF) cells since the cell viability was more than 80%

Summary

Introduction

Wound healing is a well-programmed complex process which involves inflammation, cell migration and proliferation, and neovascularization extracellular matrix production and remodeling [1]. Wound dressings form a key part of wound management to achieve rapid wound closure and minimal acceptable scar [2]. Medicinal wound dressing is an effective approach for reaching an optimum milieu for wound healing through impregnated therapeutic drugs of different pharmacological action [3, 4]. Dressings cast from hydrocolloid film are the main choices among the popular wound dressings owing to their high absorption ability via strong hydrophilic gel formation characteristics [5]. Hydrocolloid is able to absorb exudates and form gel to provide a moist environment which is essential for healing. The simple, thin with semi-permeable films permit for inspection wound condition, water vapor, and gases exchange for effective restoration of wound [6]

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