Abstract
Purpose: To investigate the healing effect of Shaoshang Yuhe yihao (SYY) in a deep second-degree burns model in rats.Methods: Female Wistar rats, weighing 200 - 220 g, were subjected to deep second-degree skin burns by electrical scald instrument. The animals were divided into three groups as follows: (1) second-degree burns model (control) group, (2) moist exposed burns ointment-treated group (positive control drug), and (3) SYY groups. The rats were treated with SYY for 21 consecutive days. The rate of healing, scabs dropped time and re-epithelialization time were observed every three days for 21 days after burns injury. Samples were collected from treated rats after sacrificing the animals on the 1st, 2nd, 5th, 9th, 14th, and 21st day post-burn creation, respectively. Plasma interleukin-1 (IL-1) levels were evaluated, and the tissue expressions of transforming growth factor-beta (TGF-β) were determined along with skin histopathology. The burned tissues were examined histologically to confirm the degree of burns and healing condition.Results: The results showed that the scabs drop time decreased (p < 0.05), and healing accelerated after treatment with SYY in the burns injury rats (94.6 ± 2.6 %, p < 0.05). Furthermore, the expressions of TGF-β significantly increased in the tissue (329.4 ± 8.2 pg/mL, p < 0.05) at day 14; However, the levels of inflammatory factors in plasma decreased (11.3 ± 1.7 pg/mL, p < 0.05). On the 21st day after burns injury, the injured tissue treated with SYY was almost (> 90 %) covered with healthy epithelial tissue and new epidermis, and showed complete epithelialization of the burns injury area.Conclusion: The results confirm the curative effects of SYY on burns injuries in rats. Thus, the herbal preparation has potentials to be developed for clinical application in burns cases.Keywords: Shaoshang Yuhe yihao, Second-degree burns, Healing, Scabs drop time, Moist exposed burns ointment
Highlights
Burn injuries, caused by heat, light, electricity, radiation and chemicals, are one of the most common and devastating forms of trauma, partial- and full-thickness burns [1].According to the report, about 5000 - 10,000 in one million people suffer from burns in China every year [2]
All the rats were randomly divided into three groups of ten rats each: deep second-degree burn model group, moist exposed burn ointment-treated group and Shaoshang Yuhe yihao (SYY)-treated group
Multiple group comparisons were performed by one-way analysis of variance (ANOVA) using SPSS 16.0 followed by Dunnett’s test to detect intergroup differences. p < 0.05 was considered statistically significant
Summary
Burn injuries, caused by heat, light, electricity, radiation and chemicals, are one of the most common and devastating forms of trauma, partial- and full-thickness burns [1]. Chinensis (Haw.) and Rheum palmatum L., is a traditional Chinese medicine for treating burns It has been used in treatment of inflammation, infection, jaundice, skin burns and hyperlipemia in China [7,8]. All the rats were randomly divided into three groups of ten rats each: deep second-degree burn model group (control), moist exposed burn ointment-treated group (positive control drug) and SYY-treated group. A series of experiments were designed to investigate the effect of SYY on burn injuries in rats. The full thickness of burn area was removed from different treated rats by sacrificing the animals on the 1st, 2nd, 5th, 9th, 14th, and 21st day post-burn creation, one aliquot (about 100 mg) was processed by standard histological techniques, and others samples were stored at 80 °C in aliquots until analysis. Multiple group comparisons were performed by one-way analysis of variance (ANOVA) using SPSS 16.0 followed by Dunnett’s test to detect intergroup differences. p < 0.05 was considered statistically significant
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