Abstract
Boiling histotripsy (BH) is an experimental non-invasive focused ultrasound (FUS) technology that uses milliseconds-long ultrasound pulses at low duty cycle to mechanically homogenize targeted tissue. Here, we report the evolution of BH lesions and the resulting immune response to in vivoablation of renal carcinoma (RCC) in a rat model. RCC bearing Eker rats and syngeneic wild-type rats were randomly assigned to transcutaneous BH or FUS SHAM procedure targeting ~0.5 cc of RCC or non-tumor bearing normal kidney. BH was delivered with a 1.5 MHz US-guided small animal FUS system (VIFU-2000, Alpinion) operated at duty cycles of 1-2%, 10-20 ms pulses, and 525-600 W electric power. Rats were survived for up to 56 days post-treatment. BH lesions evolved from sharply demarcated regions of homogenized tissue to small fibrous scars by 56 days. Compared to sham procedure, BH produced significant alterations in plasma and intrarenal cytokines and tumor/renal infiltrating leukocyte populations. These data describe the...
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