Abstract
A gas chromatographic technique for the determination of free and bound sulfur dioxide in beer was developed. Bound SO2 was decomplexed in alkaline medium in the presence of mercuric chloride. Free SO2 evolved on acidification was quantified by gas chromatography with a flame photometric detector. Sodium bisulfite was used as an external standard. The method showed good correlation with both the distillation and colorimetric methods. Recovery of SO2 and reproducibility by this gas chromatographic technique were high. Because the procedure does not use pararosaniline dye, it is safer than the colorimetric method and is equally applicable to rapid analysis of SO2 in both pale and dark beer samples.
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