Abstract
This study describes de novo transcriptome sequencing and annotation analyses for the head kidney of the sub-Antarctic notothenioid fish Eleginops maclovinus, a sister group of the Antarctic notothenioid fish clade. Moreover, E. maclovinus is one of the most eurythermal and euryhaline representatives of the Notothenioidei suborder. RNA-seq data were generated by the 454 GS Junior system, resulting in 11,207 contigs that were then assembled by the Genomic Workbench CLC software. The transcriptome was annotated by BLASTing each sequence against the universal, non-redundant NCBI database (National Center for Biotechnology Information) using the AUSTRAL-omics computer cluster. A significant number of transcripts related to innate and adaptive immunity were found in the sequences, which could be used as references in future immunological studies in E. maclovinus.
Highlights
Eleginops maclovinus (Valenciennes 1830) is a notothenioid (Perciformes) endemic to the temperate and sub-Antarctic waters of South America
The high prevalence of immune-related transcripts in the E. maclovinus head kidney is in line with previous reports from T. bernacchii [10]
The head kidney has been reported a primary lymphoid organs in the bony fishes that functions as lymphocyte precursor tissue responsible for the generation as well as maturation of B cells, and the generation of T-cell progenitor cells that migrate to the thymus throughout life [17]
Summary
Eleginops maclovinus (Valenciennes 1830) is a notothenioid (Perciformes) endemic to the temperate and sub-Antarctic waters of South America. This fish is the only representative of the Eleginopsidae (Osteichthyes) family within the Notothenioidei suborder. The Eleginopsidae, Pseudaphritidae, and Bovichtidae families emerged before a severe decrease in water temperature approximately 24 million years ago [1]. This report describes the de novo assembly and annotation of the E. maclovinus head kidney transcriptome, providing a new source of genetic information that can be used in future studies dealing with the immunological response of notothenioids
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