Abstract
The purpose is to evaluate the safety of the scleral crosslinking with riboflavin/ultraviolet A (UVA) in an experiment in vivo. Materials and methods. The study was carried out on 34 Chinchilla rabbits (68 eyes). The right eyes were crosslinked (34 eyes), the left eyes were served as controls (34 eyes). Scleral crosslinking (SCXL) was performed with sclera saturation with a photosensitizer (0.1 % aqueous solution of riboflavin) for 20 minutes and its subsequent irradiation with ultraviolet A (wavelength of 370 ± 5 nm, irradiance — 3 mW/cm2), total exposure time — 30 minutes (6 cycles of 5 minutes). The effect of the procedure on the anatomical and functional state of the eye layers was assessed with high-resolution optical coherent tomography (OCT) and electroretinography (ERG) before crosslinking, a day, 7 and 30 days after it. Results. OCT did not reveal any pathological changes after scleral crosslinking with riboflavin/UVA. The layers of the retina, choroid and sclera were clearly visualized. The performed morphometric analysis has showed the absence of statistically significant changes in the eye layers thickness after ultraviolet crosslinking. The amplitude-time characteristics and the shape of the electroretinogram of the experimental and control rabbits eyes were identical during all periods of observation, had a classic appearance. All waves of ERG were well expressed. It indicates a satisfactory functional state of the retinal neuroreceptor mechanisms. Conclusion. For ultraviolet crosslinking we used UVA with an irradiance of 3 mW/cm2 for 30 minutes and a 0.1 % aqueous solution of riboflavin without dextran and did not reveal any side effects of the procedure. Thus, scleral crosslinking with these parameters is safe for the eye layers in an experiment in vivo.
Highlights
Близорукость является одной из основных причин снижения зрения в мире
Fig. Optical coherence tomography of eye rabbits.1 day after scleral crosslinking with riboflavin/UVA: a) crosslinked group; б) control group лабораторного животного
MD, professor, leading scientific researcher of the cornea and lens surgery Department, Pushkin str., 90, Ufa, 450008, Russia
Summary
Исследование было проведено на 34 кроликах поро‐ ды Шиншилла (68 глаз). Правые глаза были опытными (34 глаза), левые служили контролем (34 глаза). Результаты измерения толщины оболочек глаза оказались следующими: толщина сетчатки кроликов опытной группы (мкм) по данным ОКТ (M ± m) до операции — 157,4 ± 7,3, через сутки после операции — 152,2 ± 6,7, через 7 дней после операции — 161,9 ± 5,7, через 30 дней после операции — 153,8 ± 7,1 мкм. Толщина хориоидеи кроликов опытной группы (мкм) по данным ОКТ (M ± m) до операции составляла 148,9 ± 7,4, через сутки после операции — 152,9 ± 7,7, че‐ рез 7 дней после операции — 143,7 ± 7,6, через 30 дней после операции — 153,1 ± 6,8 мкм. Толщина склеры кроликов опытной группы (мкм) по данным ОКТ (M ± m) до операции была 282,1 ± 8,9, через сутки после операции — 278,9 ± 9,7, через 7 дней после операции — 291,6 ± 10,2, через 30 дней после операции — 286,6 ± 9,4 мкм.
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