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Abstract
1. 1. A charon 4A human fetal liver genomic library was screened for human nonspecific alkaline phosphatase gene using the cloned human bone cDNA as a hybridization probe. 2. 2. A clone 2.2 Kb DNA was sequenced and found to contain a piece of sequences encoding the 4–44th amino acids of NH; terminus. 3. 3. The other cloned 1.6Kb DNA contains two segments of sequences each corresponding to two separate regions of the cDNA for alkaline phosphatase. The first segment of the DNA codes for the 83–141st amino acids whereas the second for 141–199th.
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