HDL 3 exerts more powerful anti-oxidative, protective effects against copper-catalyzed LDL oxidation than HDL 2

Abstract

Listen

Translate article

Objective: To evaluate which HDL subfraction, HDL 2 or HDL 3, exerts the greater preventive effect on the Cu 2+-induced LDL oxidation. Methods: LDL was incubated for 6 h with 2.5 μM Cu 2+ in phosphate-buffered saline alone, or in the presence of HDL 2 or HDL 3 at various protein concentrations. Each sample was subjected to agarose gel electrophoresis, and the amount of lipid hydroperoxide in each sample of LDL was measured. Results: There was no significant difference in the levels of LPO between the LDL and LDL + HDL 2 cases, whereas a significant reduction was apparent with LDL + HDL 3. Both HDL 2 and HDL 3 significantly inhibited oxidative modification of LDL, as assessed by electrophoretic mobility, in a concentration dependent manner, but this effect was much more pronounced with HDL 3. Conclusion: HDL 3 may play an important role in the prevention of atherosclerosis in vivo, more effectively inhibiting oxidation of LDL than HDL 2.