Abstract
In plants MADS-box transcription factors (TFs) play important roles in growth and development. However, no plant MADS-box gene has been identified to have a function related to secondary metabolites regulation. Here, a MADS-box TF gene, designated as HbMADS4, was isolated from Hevea brasiliensis by the yeast one-hybrid experiment to screen the latex cDNA library using the promoter of the gene encoding H. brasiliensis small rubber particle protein (HbSRPP) as bait. HbMADS4 was 984-bp containing 633-bp open reading frame encoding a deduced protein of 230 amino acid residues with a typical conserved MADS-box motif at the N terminus. HbMADS4 was preferentially expressed in the latex, but little expression was detected in the leaves, flowers, and roots. Its expression was inducible by methyl jasmonate and ethylene. Furthermore, transient over-expression and over-expression of HbMADS4 in transgenic tobacco plants significantly suppressed the activity of the HbSRP promoter. Altogether, it is proposed that HbMADS4 is a negative regulator of HbSRPP which participates in the biosynthesis of natural rubber.
Highlights
The MADS-box transcription factors (TFs) family is found throughout eukaryotes (Theissen et al, 2000)
In order to screen novel TFs which regulate HbSRPP, we performed yeast one-hybrid assay with the rubber tree latex cDNA library using the HbSRPP promoter as bait. 48 positive colonies were obtained and sequenced
The binding specificity of the MADS-box protein with the HbSRPP promoter was determined by the one-to-one interaction analysis
Summary
The MADS-box transcription factors (TFs) family is found throughout eukaryotes (Theissen et al, 2000). The MADS-box TFs family contains much more number of members in plants than in other eukaryotes (Grimplet et al, 2016). MADS-box TFs are best known as regulators of reproductive development, such as flowering induction, flower development, or fruit development (Theissen et al, 2000; Parenicová et al, 2003; Adamczyk et al, 2007; Arora et al, 2007; Itkin et al, 2009; Smaczniak et al, 2012a,b; Puig et al, 2013). No plant MADS-box gene has been identified to have a function related to secondary metabolites regulation
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