Abstract

The process of hatching of Aedes aegypti eggs can be resolved into two stages: (1) rupture of the water-impermeable barrier as an indirect result of decreasing oxygen concentration; and (2) entrance of water and concomitant swelling-out of the larvae from the shells. These two events can be separated in time and examined independently. Hatching was observed even when the interval between the application of stimulus and submergence was 1 week. Following stimulation the eggs may die due to dehydration. Hatching can be prevented by high osmotic pressure of the medium although the response to the stimulus occurs. Transfer of eggs from an atmosphere of pure oxygen to that of air can also serve as an effective stimulus. Action of solvents on intact eggs of A. aegypti supports the view that the water-impermeable barrier contains a lipid.

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