Abstract
In an effort to search for an efficient and environmentally friendly harvesting method, a commercially available microbial flocculant poly (γ-glutamic acid) (γ-PGA) was used to harvest oleaginous microalgae. Conditions for flocculation of marine Chlorella vulgaris and freshwater Chlorella protothecoides were optimized by response surface methodology (RSM) and determined to be 22.03mgL−1 γ-PGA, 0.57gL−1 biomass, and 11.56gL−1 salinity, and 19.82mgL−1 γ-PGA and 0.60gL−1 biomass, respectively. Application of the two optimized flocculation methods to Nannochloropsis oculata LICME 002, Phaeodactylum tricornutum, C. vulgaris LICME 001, and Botryococcus braunii LICME 003 gave no less than 90% flocculation efficiency and a concentration factor greater than 20. Micrographs of the harvested microalgal cells showed no damage to cell integrity, and hence no lipid loss during the process. The results show that flocculation with γ-PGA is feasible for harvesting microalgae for biodiesel production.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
