Abstract
BackgroundTo meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK.ResultsA new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel.ConclusionThese results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production.
Highlights
To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide
These results suggested that pongamia shell hydrolysate (PSH) and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production
In this study, 57 yeast isolates were screened for lipid accumulation by microwave-aided Nile red spectrofluorimetry
Summary
To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. Yeast strains belonging to these genera can accumulate intracellular lipids more than 60% of their dry cell weight (DCW), displayed tolerance to pre-treatment-derived inhibitors along with the ability to assimilate wide range of carbon sources [10]. To establish a sustainable microbial lipid production, extra endeavours are requisite such that yeast efficiently utilize renewable and low-cost carbon sources [12]. Inexpensive lignocellulosic carbon sources like rice straw hydrolysate [13], elephant grass hydrolysate [14], sugarcane bagasse hydrolysate [15], groundnut shell hydrolysate [16], wheat straw [17] and waste office paper hydrolysates [18] have been used for microbial lipid production. Yeast isolates utilizing C5 and C6 sugars derived from lowcost feedstock along with the potential to tolerate high concentration of pre-treatment derived inhibitors are much essential for lipid production
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