Abstract
With the aim of developing an immunoassay for the detection of the insecticide methiocarb, three compounds with carboxylic spacer arms of different lengths introduced at the carbamate group were synthesized, conjugated to proteins, and used as immunizing haptens in mice. Monoclonal antibodies (MAbs) were subsequently obtained and characterized for their affinity to methiocarb. In the homologous conjugate-coated assay format, MAbs exhibited I50 values in the 1−10 nM range. Thereafter, a collection of methiocarb haptens with modifications in the aromatic ring structure or in the spacer arm were synthesized to be used as heterologous haptens. These haptens and MAbs were incorporated into several ELISA configurations. Some of the new combinations of immunoreagents and assay format provided a significant improvement in assay sensitivity, reaching I50 values around 0.1 nM. MAbs seem to be very specific for methiocarb, as evidenced by the negligible cross-reactivity shown by methiocarb metabolites. These immunoreagents are very promising analytical tools for the rapid and sensitive determination of methiocarb in food and in the environment. Keywords: ELISA; immunoassay; insecticide; monoclonal antibodies; hapten design; hapten heterology; ELISA format; carbamate
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
