Hapten synthesis and antibody production for the development of a paper immunosensor for lean meat powder zilpaterol

Abstract

An anti-zilpaterol mAb with an IC50 of 0.31 ng mL−1 and a limit of detection (LOD) of 0.02 ng mL−1 has been developed. For semi-quantitative detection in pork samples, the visual LOD is 0.5 ng mL−1 and the cut-off value is 5 ng mL−1.