Abstract
Since hapten inhibition of precipitation is relatively time consuming, we have developed a hapten inhibition of adsorption assay to explore the sugar binding specificity of the Sophora japonica lectin. Adsorbents were prepared with Sephadex and p-aminiphenyl β-D-galactopyranoside using the CNBr procedure. The ability of simple saccharides to inhibit the binding of lectin to the adsorbent was performed employing a fixed amount of adsorbent and lectin and varying quantities of inhibitor. Plots of the percent inhibition of adsorption with respect to the logarithm of micromoles of inhibitor yielded sigmoid shaped curves. The quantities of various saccharides required to cause 50% inhibition of the lectin—adsorbent interaction relative to D-galactose were within ±5% of the relative inhibiting potencies of the sugars in the lectin—blood group substance precipitation reactions.
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