Abstract
Happily at Work
Highlights
It is a great privilege to be asked for a “Reflections” essay; I admire those prepared by my predecessors
Instead we studied a wide variety of metabolic phenomena that I have described in a summary of my first 50 years of biochemical research [2]
My students purified and crystallized some 10 phosphate-transferring enzymes, and we demonstrated that most of them required MgATP as substrate and were inhibited by free ATP; we found 16 different antibiotics that affected oxidative phosphorylation [9, 10] and a dozen that acted as ionophores [11], some of which are still being used in experiments
Summary
It is a great privilege to be asked for a “Reflections” essay; I admire those prepared by my predecessors. We had been investigating the role of calcium transport in the regulation of sperm behavior, including the acrosome reaction and enhancement of motility for some years [35, 37, 91] when Donner Babcock found that the rapid uptake of calcium by bovine epididymal sperm did not occur in sperm separated from ejaculates [38]. The obvious experiment was to add seminal fluid to epididymal sperm, which demonstrated the presence of a calcium transport inhibitor that we termed caltrin.
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