Abstract
Cell death-inducing DFFA-like effector c (CIDEC, also known as Fsp27) has emerged as an important regulator of metabolism associated with lipodystrophy, diabetes, and hepatic steatosis. It is required for unilocular lipid droplet formation and optimal energy storage. The mechanism between this gene and livestock growth traits, however, has yet to be reported. In this study, we found ten novel single nucleotide polymorphisms (SNPs) in the 5’ transcriptional region of CIDEC in Nanyang (NY) cattle, which are located in the recognition sequences (potential cis-acting elements) of 22 transcription factors, and the nine haplotypes represent nine different combinations of polymorphic potential cis-acting elements. The results indicated that individuals with the H8-H8 diplotype had heavier body weights and faster growth rates (P < 0.01) at 18th months than those with H1-H8. We evaluated the transcriptional activities of different haplotypes in vitro, the results were consistent with the association analysis. The H8 haplotype had 1.88-fold (P < 0.001) higher transcriptional activity than the H1 haplotype. We speculate that the haplotypes of the potential cis-acting elements may affect the transcriptional activity of CIDEC, thus affecting the growth traits of cattle. This information may be used in molecular marker-assisted selection of cattle breeding in the future.
Highlights
Cell death-inducing DFFA-like effector c (CIDEC, known as fat-specific protein 27 (Fsp27)) has emerged as an important regulator of metabolism associated with lipodystrophy, diabetes, and hepatic steatosis
We found nine potential cis-acting element haplotypes involving ten novel variations in the bovine CIDEC gene, and we assessed the genetic impacts of these haplotypes on bovine body weight (BW) and average daily gain (ADG)
Fsp27/leptin double-deficient mice were resistant to diet-induced obesity and displayed increased insulin sensitivity[8,10]
Summary
Cell death-inducing DFFA-like effector c (CIDEC, known as Fsp27) has emerged as an important regulator of metabolism associated with lipodystrophy, diabetes, and hepatic steatosis. It is required for unilocular lipid droplet formation and optimal energy storage. We speculate that the haplotypes of the potential cis-acting elements may affect the transcriptional activity of CIDEC, affecting the growth traits of cattle. This information may be used in molecular marker-assisted selection of cattle breeding in the future. The results of this research will contribute to the understanding of the regulatory mechanisms of the CIDEC gene, and may be used in molecular marker-assisted selection of live cattle breeding in the future
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