Haplotypes association of [-794(CATT)5-8/-173G>C] MIF gene polymorphism with Acute Coronary Syndrome

Abstract

Event Abstract Back to Event Haplotypes association of [-794(CATT)5-8/-173G>C] MIF gene polymorphism with Acute Coronary Syndrome Emmanuel V. Alvarado1, José F. Muñoz-Valle2, Elena Sandoval Pinto1, Ilian Janet G. González1, Angélica V. Haro1 and Jorge Ramón P. Gutierrez2* 1 Estudiante de doctorado, Mexico 2 Universidad de Guadalajara, Mexico Introduction: Acute coronary syndrome (ACS) is characterized by acute, regional reductions in coronary blood flow and myocardial ischemia. ACS describes a spectrum of clinical manifestations, including unstable angina (UA), non-ST-segment elevation myocardial infarction (NSTEMI) and ST-segment elevation myocardial infarction (STEMI). The vast majority of ACS are triggered by disruption of an atherosclerotic plaque. The macrophage migration inhibitory factor (MIF) is related to the progression of atherosclerosis, which, in turn, is a key factor in the development of acute coronary syndrome (ACS). MIF has a single nucleotide polymorphism at position -173 G>C and a CATT short tandem repeat (STR) at position -794 that might be involved in its expression rate. The aim of this study was to investigate the association of the haplotypes [-794(CATT)5-8/-173G>C] of MIF gene polymorphism with ACS in a western Mexican population. Methods: This research included 200 ACS patients classified according to the criteria of the American College of Cardiology (ACC) and 200 healthy subjects (HS). The -794 (CATT)5-8 MIF gene polymorphism was analyzed using a conventional polymerase chain reaction (PCR) technique and The -173 G>C MIF polymorphism was genotyped by the PCR restriction fragment length polymorphism (RFLP) technique. The Hardy-Weinberg equilibriumtest and genotype and allele frequencies were calculated by the chi-square test or Fisher’s exact test, when applicable.Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated to test the probability that the genotype and allele frequencies were associated with ACS. A □□ value <0.05 was considered to be statistically significant. All the statistical analyses were done with the Stata 9.0 software. Results: The haplotype 6G was the most frequent in both groups (ACS: 51% and HS: 47%). A significant association was found between the 7G haplotype and susceptibility to ACS (3% vs 6% in ACS and HS respectively, p = 0.04). Conclusion: We conclude that the 7G haplotype of the MIF gene polymorphism is associated with susceptibility to ACS in a Western Mexican population. Acknowledgements The authors greatly appreciate the participation of every person in this study Keywords: ACS, Atherosclerosis / CAD, MIF, MIF HAPLOTYPES, Cardiovascular Diseases Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Oral Presentation Topic: Immunotherapy Citation: Alvarado EV, Muñoz-Valle JF, Sandoval Pinto E, González IG, Haro AV and Gutierrez JP (2015). Haplotypes association of [-794(CATT)5-8/-173G>C] MIF gene polymorphism with Acute Coronary Syndrome. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00320 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 May 2015; Published Online: 15 Sep 2015. * Correspondence: Dr. Jorge Ramón P Gutierrez, Universidad de Guadalajara, Guadalajara Jalisco, Mexico, imey_99@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Emmanuel V Alvarado José F Muñoz-Valle Elena Sandoval Pinto Ilian Janet G González Angélica V Haro Jorge Ramón P Gutierrez Google Emmanuel V Alvarado José F Muñoz-Valle Elena Sandoval Pinto Ilian Janet G González Angélica V Haro Jorge Ramón P Gutierrez Google Scholar Emmanuel V Alvarado José F Muñoz-Valle Elena Sandoval Pinto Ilian Janet G González Angélica V Haro Jorge Ramón P Gutierrez PubMed Emmanuel V Alvarado José F Muñoz-Valle Elena Sandoval Pinto Ilian Janet G González Angélica V Haro Jorge Ramón P Gutierrez Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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