Haplotype Structures of Common Variants ofCYP2C8,CYP2C9, andADRB1Genes in a South Indian Population

Abstract

In association with candidate genes, the observed trait may be due to either one of the variant alleles or the interaction of variant alleles at different loci, which are in linkage disequilibrium. The objective of this study was to investigate the baseline allele and genotype frequencies, linkage disequilibrium (LD) patterns, and haplotype structures of common variants of the CYP2C8, CYP2C9, and ADRB1 genes located on chromosome 10. Two hundred and forty-five healthy subjects were recruited from South India and were compared with the HapMap Project's population for LD pattern, allele and genotype frequencies, and haplotype structures. Genotyping was done using polymerase chain reaction-restriction fragment length polymorphism and TaqMan assay on real-time polymerase chain reaction. A significant ethnic difference was found in the LD patterns among the variant alleles between the South Indian population and other major ethnic groups, namely African, European, Chinese, and Japanese. This study established the normative allele and genotype frequencies, haplotype structure, and LD patterns of common variants of the CYP2C8, CYP2C9, and ADRB1 genes in a South Indian population (Tamilian). The data may be helpful to plan candidate gene-trait association studies in this population.