Haplotype Frequencies for Two New Y-STR Loci in Chinese Population

Abstract

Abstract Blood samples were collected from 104 unrelated male individuals of Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). Primers for Y-GATA-C4 were redesigned by us. The sequences of the primers for Y-GATA-C4 were 5' -gtggaaccagcccaaatatc-3' and 5' -aatgctctcttggcttctcact-3'. Primers for Y-GATA-A10 were in accordance with White's (2). The PCR amplification conditions can be obtained at: http://www.legalmed.org/dna/y-c4a10.htm. The PCR reaction volume for each locus was 37.5 μL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (3). The diversity of haplotype, the discrimination power and the probability of exclusion for the two Y-STR loci were calculated according to Hou's method (4).