Hansschlegelia

Abstract

Abstract Hans.schle.ge'li.a. N.L. fem. n. Hansschlegelia , of Hans Schlegel, named after the famous German microbiologist, Professor Hans G. Schlegel, known for his classic studies on autotrophic bacteria. Pseudomonadota / Alphaproteobacteria / Rhizobiales / Methylopilaceae / Hansschlegelia The genus Hansschlegelia is represented by Gram‐negative, nonsporulating, colorless, and nonmotile or motile short rod‐shaped bacteria that occur singly or in pairs. Colonies are white to light pink, translucent, convex, round, and up to 2 mm in diameter. Obligate aerobes, having a strict respiratory type of metabolism with oxygen as the terminal electron acceptor. Reduce nitrates to nitrites. Restricted facultative methylotrophs. Assimilate C 1 compounds via the isocitrate lyase‐negative serine pathway. Utilize only several polycarbon substrates. Growth occurs on a mineral salt medium with methanol, formate, and glycerol; some strains can utilize methylamine. Growth factors are not required. Nitrogen sources include methylamine, amino acids, ammonium salts, and nitrates. Methyl red‐ and Voges–Proskauer‐negative. Urease‐, catalase‐, and oxidase‐positive. Able to form indole from l ‐tryptophan on mineral medium with methanol and KNO 3 . Neutrophilic and mesophilic. Growth at pH 5.0–9.0 and within a temperature range between 10 and 40°C. No growth in the presence of 2% NaCl (w/v). The major ubiquinone is Q‐10. The dominant phospholipids are phosphatidylethanolamine, phosphatidylcholine, and diphosphatidylglycerol. The prevailing cellular fatty acids are C 18:1 ω7 c , C 16:0 , and C 19:0 cyclo acids. The genome sizes range between 3.58 and 4.62 Mb. According to Genome Taxonomy Database (GTDB), the genus Hansschlegelia belongs to the phylum Pseudomonadota , class Alphaproteobacteria , order Rhizobiales , and family Methylopilaceae . The type species is Hansschlegelia plantiphila . Representatives of this genus inhabit phyllosphere and rhizosphere of various plants and rhizospheric and polluted farmland soils. DNA G + C content (mol%) : 67.0–69.4 (genome sequence). Type species : Hansschlegelia plantiphila Ivanova et al. 2007, VL133.