Hamster Sperm-Associated Alpha-l-Fucosidase Functions During Fertilization

Abstract

Sperm-associated alpha-L-fucosidases have been identified in diverse organisms. Their wide phylogenetic distribution and known properties support the likelihood that L-fucose and alpha-L-fucosidase have fundamental function(s) during gamete interaction. This is consistent with the substantial evidence in the literature documenting the importance of carbohydrate moieties during fertilization. Direct enzyme assays were employed to evaluate the functional distribution of alpha-L-fucosidase in preparations of hamster sperm. In vitro fertilization was performed using Syrian hamster sperm and eggs to identify the functional role of hamster sperm-associated alpha-L-fucosidase during zona pellucida binding/penetration, sperm-egg membrane fusion, and postfusion events. Results reported here document the presence of hamster sperm-associated alpha-L-fucosidase and demonstrate that it functions during fertilization at the stage of sperm-oocyte membrane interaction and/or postfusion events within the zygote. Understanding the role of alpha-L-fucosidase during human fertilization could lead to development of improved infertility treatments.