Halorubrum salipaludis sp. nov., isolated from the saline-alkaline soil.

Qi Gong,Pu Zhao,Keke Yi,Shaohua Miao,Guishan Zhang,Chunhong Ma

doi:10.1007/s00203-021-02729-1

Abstract

A novel extremely halophilic archaeon, designated WN019T, was isolated from the natural saline-alkali wetland soil of Binhai new district, Tianjin, China. Cells of WN019T were aerobic, motile, and pleomorphic rod-shaped, 0.5-0.8µm in width and 2.0-2.5µm in length, and the growth occurred optimally at 33-37°C, pH 7.5-8.0, and in the presence of 15.0-20.0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequence comparison showed that the isolate belonged to the genus Halorubrum and exhibited moderate sequence similarity of 97.8% to Halorubrum saccharovorum JCM 8865T. The major respiratory quinones of strain WN019T were MK-8 and MK-8 (H2), and the major polar lipids were glycolipid (GL), phospholipid (PL), phosphatidylglycerol-sulphate (PGS), phosphatidylglycerol (PG) and phosphatidylglycerol-phosphate-methyl ester (Me-PGP). The DNA G + C content of the strain was 67.4mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) value based on whole genome sequences of strain WN019T and Halorubrum saccharovorum JCM 8865T were 87.5% and 35.4%, respectively. Phenotypic, chemotaxonomic, phylogenetic, and genomic analyses suggested that strain WN019T represents a novel species of the genus Halorubrum, for which the name Halorubrum salipaludis sp. nov. is proposed. The type strain is WN019T (= KCTC 4269T = ACCC 19977T).

Highlights

The genus Halorubrum was first proposed by McGenity and Grant belonging to the family Halorubraceae, and the type strain of Halorubrum saccharovorum was affiliated to strain JCM 8865T (McGenity and Grant 1995)
At the time of writing, the genus Halorubrum, which is the largest genus within the family Halorubraceae, consists of 37 validly published species that are phylogenetically and phenotypically heterogeneous listed on the LPSN
We reported the novel strain WN019T of the genus Halorubrum, which can live in the saline-alkaline habitat, according to their phenotypic, biochemical, and genotypic characteristics

Summary

Introduction

The genus Halorubrum was first proposed by McGenity and Grant belonging to the family Halorubraceae, and the type strain of Halorubrum saccharovorum was affiliated to strain JCM 8865T (McGenity and Grant 1995). At the time of writing, the genus Halorubrum, which is the largest genus within the family Halorubraceae, consists of 37 validly published species that are phylogenetically and phenotypically heterogeneous listed on the LPSN (http://www.bacterio.net/). It has been reported that the genus Halorubrum are widely distributed in various hypersaline environments, such as salt lakes, hypersaline soda lakes, coastal sabkhas, salt mine, saline soil, salt-fermented seafood, and marine salterns (Chen et al 2017a, b; de la Haba et al 2018; Ochsenreiter et al 2002). Halorubrum species, which grow fast and are active in the conversion of different kinds of substrates, may play a major role in carbon and nitrogen cycling of several hypersaline environments (Feng et al 2004). We reported the novel strain WN019T of the genus Halorubrum, which can live in the saline-alkaline habitat, according to their phenotypic, biochemical, and genotypic characteristics

Methods

Results

Conclusion