Halopelagius fulvigenes sp. nov., a halophilic archaeon isolated from a lake

Abstract

Two extremely halophilic archaea, designated YIM 94188(T) and YIM 94189, were isolated from Qijiaojing lake in Xinjiang province, north-west China and subjected to taxonomic characterization using a polyphasic approach. The cells of the two strains were coccoid, non-motile and Gram-stain-negative. Colonies were pink-white-pigmented and aerobic. Growth occurred at 10-30% (w/v) NaCl, 20-55 °C and pH 6.0-8.0 (optimum: 20-25% NaCl, 37-42 °C, pH 6.5-7.0). Magnesium was necessary for growth in the range of 0.2-1.2 M. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains belonged to the genus Halopelagius showing 98.5% sequence similarity to the closest phylogenetic neighbour, Halopelagius inordinatus RO5-2(T). In addition, the DNA-DNA hybridization values of strains YIM 94188(T) and YIM 94189 to Halopelagius inordinatus RO5-2(T) were 35.7% and 37.7%, respectively. Polar lipid analyses revealed that the two strains contained phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The DNA G+C contents of strains YIM 94188(T) and YIM 94189 were 66.3 mol% and 64.6 mol%, respectively. On the basis of physiological and chemotaxonomic data, and phylogenetic analysis, strains YIM 94188(T) and YIM 94189 were classified as representing a novel species in the genus Halopelagius. The name Halopelagius fulvigenes sp. nov. is proposed, with YIM 94188(T) (=CCTCC AB 2010456(T)=JCM 17506(T)) as the type strain.