Abstract
Strain WRN001T, a Gram-staining-negative, strictly aerobic, non-motile bacterium was isolated from the natural saline-alkali wetland soil of Binhai new district, Tianjin, China (38°46' N, 117°13' E). Cells of strain WRN001T were 0.3-0.5µm in width and 1.5-2.5µm in length, and the growth occurred optimally at 33-37°C, pH 7.5-8.0, and in the presence of 8-10% (w/v) NaCl. Based on 16S rRNA gene sequence analysis, the isolate could be affiliated to the genus Halomonas, and the highest 16S rRNA gene sequence similarity of strain WRN001T to its closest relative Halomonas qiaohouensis DSM 26770T was 97.5%. The size of the genome as presented here was 5,475,884bp with a G + C content of 63.8mol %. The major respiratory quinone of strainWRN001T was Q-9, and the dominant fatty acids were summed feature 8, summed feature 3, C10:0, C12:0, C12:0 3-OH, C16:0, and C17:0 cyclo. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phophatidylcholine (PC), two phospholipids (PL), aminolipid (AL), and three unidentified lipids (L). These data combined with the low digital DDH values between strain WRN001T and the close relative, Halomonas alkalitolerans CGMCC 1.9129T (42.2%) and based on comparisons with currently available genomes, the highest average nucleotide identity (ANIm) value was 91.4% to Halomonas alkalitolerans CGMCC 1.9129T (GenBank accession No. GCA_001971685.1). Therefore, we propose a novel species in the genus Halomonas to accommodate this novel isolate: Halomonas salipaludis sp. nov. (type strain WRN001T = KCTC 52853T = ACCC 19974T).
Highlights
We described the isolation, identification and physio-biochemical characteristics of novel strain WRN001T and proposed the name Halomonas salipaludis for this bacterium
Cell morphology was assessed by transmission electron microscopy (TEM), i.e., cells were harvested from exponentially growing culture, and the cells were negatively stained with 0.5 % uranyl acetate and the grids were examined at the microscope (Tecnai Spirit, FEI, Hillsboro, OR, USA)
The novel isolate WRN001T can utilize starch, casein and Tween 80, but gelatin was not used as carbon source, and nitrate was not reduced, and strain WRN001T cannot grow under anaerobic conditions
Summary
The Halomonas as a large genus was firstly described by Vreeland et al (1980), with Halomonas elongata as the type species, and most of the species of the genus Halomonas were isolated from saline habitats, such as sea sediments, salt lakes, brines, salty foods, deep sea hydrothermal vent environments, as well as saline sand and soils (Arenas et al 2009; Cao et al 2013; Dou et al 2015; Guan et al 2010; Guzmán et al 2010; Kaye et al 2004; Kim et al 2010; Lee et al 2005; Reddy et al 2003; Romano et al 1996; Wang et al 2012; Wang et al 2014; Xu et al 2007). At the time of writing, the genus Halomonas includes 96 species with validly published names (http://www.bacterio.net/halomonas.html). Some of them have been recognized for potential use in biotechnology, such as in the production of bioactive compounds (Wang et al 2006), extreme-enzymes (Kumar et al 2012), exopolysaccharide (Kumar et al 2012; Martínezcheca et al 2005; Poli et al 2013), and hydrolytic enzymes (Sánchez-Porro et al 2003). We described the isolation, identification and physio-biochemical characteristics of novel strain WRN001T and proposed the name Halomonas salipaludis for this bacterium
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
