Abstract
Ibotenic acid and muscimol are considered the principal components responsible of the hallucinogenic properties of Amanita muscaria. In this paper a sensitive ion-interaction HPLC method is presented which permits the simultaneous determination of ibotenic acid and muscimol. A C18 reverse phase is the stationary phase and the mobile phase is an aqueous solution of 5.0 mM octylammonium o-phosphate. A spectrophotometric comparative detection performed at 230 and 254 nm is employed. Detection limits are of 18 μg/L for ibotenic acid and of 30 μg/L for muscimol.
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