Half-wet nanomechanical sensors for cellular dynamics investigations

Abstract

Testing devices based on cell tracking are particularly interesting as diagnostic tools in medicine for antibiotics susceptibility testing and in vitro chemotherapeutic screening.In this framework, the application of nanomechanical sensors has attracted much attention, although some crucial aspects such as the effects of the viscous damping, when operating in physiological conditions environment, still need to be properly solved. To address this problem, we have designed and fabricated a nanomechanical force sensor that operates at the interface between liquid and air.Our sensor consists of a silicon chip including a 500 μm wide Si3N4 suspended membrane where three rectangular silicon nitride cantilevers are defined by a lithographically etched gap. The cantilevers can be operated in air, fully immersed in a liquid environment and in half wetting condition, with one side in contact with the solution and the opposite one in air. The formation of a water meniscus in the gap prevents the leakage of medium to the opposite side, which remained dry and is used to reflect a laser to measure the cantilever deflection. This configuration enables to keep the cells in physiological environment while operating the sensor in dry conditions.The performance of the sensor has been applied to monitor the motion and measures the forces developed by migrating breast cancer cell. The functionalization of one side of the cantilever and the use of a purposely designed chamber of measurements enable the confinement of the cell only on one side of the cantilever.Our data demonstrate that this approach can distinguish the adhesion and contraction forces developed by different cell lines and may represents valuable tool for a fast and quantitative in-vitro screening of new chemotherapeutic drugs targeting cancer cell adhesion and motility.