Haldina cordifolia (Roxb.) Ridsdale Bark Derived Synthesis and Characterization of Silver Nanoparticles: Investigation of its Antituberculosis and Anticancer Activity

Abstract

Nanotechnology is a rapidly growing field of medical research that may be used to a broad array of applications. Further, phytoconstituents are a promising possibility for the production of silver nanoparticles (AgNPs). AgNPs were biosynthesized using Haldina cordifolia bark extract and further characterization was done using UV-Vis Spectrophotometer, FTIR, XRD, DLS, Zeta potential and HR-TEM with SAED. The cytotoxic effect on lung cancer cell lines (A549) of AgNPs was studied by MTT assay and its antituberculosis activity was evaluated by MABA assay. The result showed that the AgNPs synthesized using bark extract of Haldina cordifolia have reported visible reddish-brown color and the absorption peak at 408nm (pH 10.5). FTIR confirmed the plant secondary metabolites that helped in green synthesis and capping of AgNPs. XRD revealed the crystalline nature of AgNPs. Zeta potential showed the stability at -42mV. HR-TEM and DLS images of AgNPs indicated the size ranged from 7 to 57nm. The dose response dependent anticancer activity was observed with IC50 value 17.64 µg/ml on A549 cell line which was more efficient than plant extract (IC50=79.92 µg/ml). On the other hand, AgNPs also showed promising antituberculosis activity with the MIC 0.4 µg/ml which was higher than the standard drugs used for comparison. We conclude that Haldina cordifolia is considered as an effective reducing and capping agent for green synthesis of AgNPs. Phytosynthesized AgNPs may subsequently be potential chemopreventive agent against the cancer treatment and as antituberculosis agent in future.