Abstract
In the present study, some pea mutants rich in protein with a modified morph leaf type with af and tl genes were genetically transformed using different strains of Agrobacterium rhizogenes A-4, A-48, 15834. Even though a higher total protein content was obtained with the A-48 strain, maximum of the probable transformants were obtained with the A-4 strain but of transient character. The content of the total protein in hairy roots cultures, depending on the composition of the nutrient media and the growth phase of the culture, has been refined.
Highlights
A promising breeding and technological element now is the genetic transformation using Agrobacterium rhizogenes, as evidenced by the large flow of literature in this direction [1, 2, NASA Astrophysics Data System (ADS)]
The following were the materials used in the study: modified morph leaf type mutants with af and tl genes and usual leaf morph varieties and lines of the Transdniestrian Agricultural Research Institute
The results revealed the genotypic component of the total protein content in mutants with different leaf morph type in stable and controlled conditions
Summary
A promising breeding and technological element now is the genetic transformation using Agrobacterium rhizogenes, as evidenced by the large flow of literature in this direction [1, 2, NASA Astrophysics Data System (ADS)]. The resulting hairy roots, subcultured on hormon free media, can be used in various areas of production, for example, for the manufacture of fast-decomposing to CO2 and H2O packaging material [3], in feed production as a valuable protein additive. The use of hairy roots as a raw material for the production of new types of drugs has every reason to transform into a new industry [4]. The goal of our research is genetic transformation of some pea mutants by TDNA A. rhizogenes with Ri plasmids. Research objectives: receiving hairy roots as a potential forage product; obtaining the pea stock material with new economic and valuable characters
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